I think, when a puppy starts getting pretty big, then it would be time to stop that behavior. You just would not be carrying a 30 pound puppy by the scruff of their neck, you wouldnt, that can really be painful. I am sure, also, it is the same way in the wild. I do know that the smaller the dog, the more puppy-ish behavior they keep. For example, Titus still has that sweet baby kind of smell to him, he still has a lot of extra skin, a domed head, soft eyes, all puppy traits. A 60lbs dog is unlikely to have that extra skin for one and for two you would almost be tearing their skin off if you picked them up like such.
Im kind of rambling, but my theory would be that the more weight on the dog, the less likely it is to be a good idea to pick them up at their scruff. Ten pounds = okay; 40 pounds = probably not.
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